| Clone ID | DM44 |
|---|---|
| Target | |
| Synonyms | CD48; BCM1; SLAMF2; BLAST; BLAST1; MEM-102; TCT.1; BCM-1; SLAMF-2; BLAST-1 |
| Host Species | Rabbit |
| Description | Anti-CD48 antibody(DM44); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P09326 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt; WB |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100; WB 1:1000 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells; dendritic cells and endothelial cells; and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain; however; but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-CD48 antibody(DM44), Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD48 (right) were surface stained with Rabbit anti-CD48 monoclonal antibody 1μg/ml ( clone: DM44) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD48 monoclonal antibody ( clone: DM44) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Flow cytometry analysis of antigen binding of rabbit anti-human CD48 mAb(DME100043).
(A) DME100043 does not bind to 293T cells that do not express CD48.
(B) A clear peak shift of DME100043 was seen compared to the control when incubated with CD48-expressing Raji cells, indicating strong binding of DME100043 to CD48. Antibodies were incubated at 2 μg/mL.
Figure 5. Anti-CD48 antibody (SKU# DME100043) at 1/1000 dilution
Lane : Raji (human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 28 kDa
Observed band size: 40 kDa
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