| Clone ID | DM82 |
|---|---|
| Target | |
| Synonyms | HAVCR2; TIM3; TIMD3; FLJ14428; KIM3 |
| Host Species | Rabbit |
| Description | Anti-TIM3 antibody(DM82); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | Q8TDQ0 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene belongs to the immunoglobulin superfamily; and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions; whereas; Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation; and inhibits Th1-mediated auto- and alloimmune responses; and promotes immunological tolerance. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-TIM3 antibody(DM82); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human TIM3 protein, mFc-His tagged protein PME100030 can bind Rabbit anti-TIM3 monoclonal antibody ( clone: DM82) in a linear range of 0.32-40 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human TIM3 (B) were surface stained with Rabbit anti-TIM3 monoclonal antibody 1μg/ml ( clone: DM82) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry analysis of antigen binding of rabbit anti-human TIM3 mAb(DME100082).
(A) DME100082 does not bind to 293T cells that do not express TIM3.
(B) A clear peak shift of DME100082 was seen compared to the control when incubated with TIM3-expressing 8226 cells, indicating strong binding of DME100082 to TIM3. Antibodies were incubated at 10 μg/mL.
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