| Clone ID | DM152 |
|---|---|
| Target | |
| Synonyms | CD126; gp80; IL-6R-1; IL-6RA; IL6Q; IL6RA; IL6RQ |
| Host Species | Rabbit |
| Description | Anti-IL6R antibody(DM152); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P08887 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST:GP130:IL6-beta); a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases; such as multiple myeloma; autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-IL6R antibody(DM152); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human IL6R protein, His tagged protein PME100109 can bind Rabbit anti-IL6R monoclonal antibody (clone: DM152) in a linear range of 1-100 ng/ml.
Figure 2. Flow cytometry analysis with Anti-IL-6R (DM152) on Expi293 cells transfected with human IL-6R (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 3. Flow cytometry analysis of antigen binding of rabbit anti-human IL6R mAb(DME100152).
(A) DME100152 does not bind to CHO-S cells that do not express IL6R.
(B) A clear peak shift of DME100152 was seen compared to the control when incubated with IL6R-expressing THP-1 cells, indicating strong binding of DME100152 to IL6R. Antibodies were incubated at 5 μg/mL.
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