| Clone ID | DM171 |
|---|---|
| Target | |
| Synonyms | CAIX; MN |
| Host Species | Rabbit |
| Description | Anti-CA9 antibody(DM171); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | Q16790 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes; including respiration; calcification; acid-base balance; bone resorption; and the formation of aqueous humor; cerebrospinal fluid; saliva; and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma; but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization; however; radiation hybrid mapping localized it to 9p13-p12. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-CA9 antibody(DM171), Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Flow cytometry analysis with Anti-CA9 (DM171) on Expi293 cells transfected with human CA9 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2. Flow cytometry analysis of antigen binding of rabbit anti-human CA9 mAb(DME100171).
(A) DME100171 does not bind to Jurkat cells that do not express CA9.
(B) A clear peak shift of DME100171 was seen compared to the control when incubated with CA9-expressing 8226 cells, indicating strong binding of DME100171 to CA9. Antibodies were incubated at 5 μg/mL.
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