| Clone ID | DM204 |
|---|---|
| Target | |
| Synonyms | CD73;NT5E;5'-Nucleotidase;5'-NT;NT5;NTE |
| Host Species | Rabbit |
| Description | Anti-CD73 antibody(DM204); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P21589 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq; Mar 2011] |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-CD73 antibody(DM204); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Flow cytometry analysis with Anti-CD73 (DM204) on Expi293 cells transfected with human CD73 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2. Flow cytometry analysis of antigen binding of Rabbit anti-human CD73 mAb(DME100204).
(A) DME100204 does not bind to Jurkat cells that do not express CD73.
(B) A clear peak shift of DME100204 was seen compared to the control when incubated with CD73-expressing HT55 cells, indicating strong binding of DME100204 to CD73. Antibodies were incubated at 10 μg/mL.
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