| Clone ID | DM212 |
|---|---|
| Target | |
| Synonyms | CD47; MER6; IAP; OA3 |
| Host Species | Rabbit |
| Description | Anti-CD47 antibody(DM212); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | Q08722 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3; Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets; and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA; binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion; enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-CD47 antibody(DM212); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Competition assay demonstrating DM212 blockade of SIRPα binding to Jurkat cell line.
Figure 2. Flow cytometry analysis of antigen binding of rabbit anti-human CD47 mAb(DME100212).
(A) DME100212 does not bind to CHO-S cells that do not express CD47.
(B) A clear peak shift of DME100212 was seen compared to the control when incubated with CD47-expressing SNU-5 cells, indicating strong binding of DME100212 to CD47. Antibodies were incubated at 5 μg/ml.
Figure 3. Flow cytometry analysis of antigen binding of rabbit anti-human CD47 mAb(DME100212).
(A) DME100212 does not bind to CHO-S cells that do not express CD47.
(B) A clear peak shift of DME100212 was seen compared to the control when incubated with CD47-expressing 8226 cells, indicating strong binding of DME100212 to CD47. Antibodies were incubated at 5 μg/ml.
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