| Tag | C-Flag Tag |
|---|---|
| Target | |
| Synonyms | B1; Bp35; CVID5; FMC7; LEU-16; MS4A1; S7 |
| Description | Human CD20 full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | P11836 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Hematopoietic cell lineage |
| Molecular Weight | The human full length CD20 protein has a MW of 33.1 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | A member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human CD20 full length protein-synthetic nanodisc
Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00
Product Data
Images
Figure1. Elisa plates were added with Flag Tag CD20-Nanodisc (0.2μg/per well) on an anti-Flag monoclonal antibody pre-coated (0.5μg/per well) plate. Serial diluted anti-CD20 monoclonal antibody (BME100160) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CD20 monoclonal antibody binding with CD20-Nanodisc is 514.4ng/ml.
Figure2. Human CD20-Nanodisc, Flag Tag on SDS-PAGE
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