Clone ID | DMC494 |
---|---|
Target | |
Synonyms | CD32; CD32A; CDw32; FCG2; FcGR; FCGR2; FCGR2A1; IGFR2 |
Host Species | Rabbit |
Description | Anti-CD32a antibody(DMC494); IgG1 Chimeric mAb |
Delivery | In Stock |
Uniprot ID | P12318 |
IgG type | Rabbit/Human Fc chimeric IgG1 |
Clonality | Monoclonal |
Reactivity | Human |
Applications | Flow Cyt |
Recommended Dilutions | Flow Cyt 1:100 |
Purification | Purified from cell culture supernatant by affinity chromatography |
Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils; and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq; Oct 2008] |
Usage | Research use only |
Conjugate | Unconjugated |
Anti-CD32a antibody(DMC494); IgG1 Chimeric mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Flow cytometry analysis with Anti-CD32a (DMC494) on Expi293 cells transfected with human CD32a (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2. Flow cytometry analysis of antigen binding of anti-human CD32a mAb(DMC100494).
(A) DMC100494 does not bind to CHO-S cells that do not express CD32a.
(B) A clear peak shift of DMC100494 was seen compared to the control when incubated with CD32a-expressing THP-1 cells, indicating strong binding of DMC100494 to CD32a. Antibodies were incubated at 5 μg/mL.
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