Case Study on Membrane Protein

DIMA Biotech offers a wide range of protein products made from mammalian cell expression systems. With a catalog of over 1000 off-the-shelf functional validated proteins, we are committed to providing high-quality and reliable research tools to scientists and researchers around the world.

All proteins are shipped as lyophilized (freeze-dried) proteins. This preservation method not only allows for long-term storage but also maintains the stability and activity of the proteins upon reconstitution. Scientists can have confidence in the quality and reliability of DIMA Biotech’s protein products, knowing that they will perform consistently in their experiments.

Extracellular domain (ECD)
Full-length Membrane Proteins

For single pass membrane proteins, DIMA offers Extracellular Domain (ECD) proteins that demonstrate exceptional activities and stabilities. These proteins have been extensively tested and validated to ensure their functional integrity, making them ideal for various applications in drug discovery and development.

High Purity, High Bioactivity

Protein Purity

Figure 1. Human SIRPa, hFc-His Tag (A) and CD47, mFc-His Tag (B) on SDS-PAGE under reducing condition.

Receptor-ligand interaction

Figure 2.
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD47, mFc-His tagged protein (PME100008) can bind its native ligand Human SIRPα, hFc-His tagged protein (PME100009) in a linear range of 3.3-26.37 ng/ml.

Biosimilar interaction

Figure 3.
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD47, mFc-His tagged protein (PME100008) can bind Anti-CD47 (Neutralizing antibody clone Hu5F9-G4) (BME100001) in a linear range of 0.061-1.606 ng/ml.

Freeze-thaw stability testing

Protein Purity Lane 1: Human SIRPα, hFc-His tagged protein without freeze-thaw treatment, Lane 2: Human SIRPα, hFc-His tagged protein after one freeze-thaw cycle, Lane 3: Human SIRPα, hFc-His tagged protein after three freeze-thaw cycles, Lane 4: Human SIRPα, hFc-His tagged protein after five freeze-thaw cycles.

Receptor-ligand interaction A: Human SIRPα, hFc-His tagged protein without freeze-thaw treatment. B: Human SIRPα, hFc-His tagged protein after one freeze-thaw cycle. C: Human SIRPα, hFc-His tagged protein after three freeze-thaw cycles. D: Human SIRPα, hFc-His tagged protein after five freeze-thaw cycles.

Storage Stability: Shelf Life Testing

ACE2-hFc, 37℃, 1、2、3 days storage stability

Lane 1: Human ACE2, hFc tagged protein after storage at -20℃ ； Lane 2: Human ACE2, hFc tagged protein after storage at 37℃ after 24h； Lane 3: Human ACE2, hFc tagged protein after storage at 37℃ after 48h； Lane 4: Human ACE2, hFc tagged protein after storage at 37℃ after 72h.

ELISA plate pre-coated by 2 μg/ml (100 μl/well) S-RBD, mFc-His tagged protein (PME100497) can bind Human ACE2, hFc Tagged protein (Stored at -20℃ or 37℃ for 24h, 48h and 72h) in a linear range of 0.488-49.83 ng/ml.

CCR8-hFc, 37℃, 1、2、3 days storage stability

Lane 1: Human CCR8, hFc tagged protein after storage at -20℃ ； Lane 2: Human CCR8, hFc tagged protein after storage at 37℃ after 24h； Lane 3: Human CCR8, hFc tagged protein after storage at 37℃ after 48h； Lane 4: Human CCR8, hFc tagged protein after storage at 37℃ after 72h.

ELISA plate pre-coated by 1μg/ml (100μl/well) Human CCR8, hFc Tag ( (Stored at -20℃ or 37℃ for 24h, 48h and 72h) ) can bind Anti-CCR8 Neutralizing antibody (BME100063) in a linear range of 3.2-80 ng/ml.

Storage Stability Testing After Reconstitution

37℃, 1、2、3 days storage stability after reconstitution of lyophilized protein

SDS-PAGE

ELISA

PME100073：Human ACE2 Protein, hFc Tag

SDS-PAGE

ELISA

PME101091：Human CCR8 Protein, hFc Tag

Lot-to-lot consistency

CCR8-hFc lot-to-lot consistency

Different batches of Human CCR8 Protein, hFc Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 1μg/ml (100μl/well) different batches Human CCR8, hFc Tag (PME101091) can bind Anti-CCR8 Neutralizing antibody (BME100063) in a linear range of 3.2-80 ng/ml.

Full length membrane proteins are often unstable and rapidly lose activity after solubilization in buffer. DIMA Biotech has pooled expertise in the cutting-edge technologies of membrane proteins and developed several development platforms for different proteins. Unlike other full length transmembrane proteins, DIMA’s proteins have shown high activities and great stabilities even after lyophilization and in the accelerated testing.