| Clone ID | DM69 |
|---|---|
| Target | |
| Synonyms | CD244;2B4;SLAMF4;NKR2B4;NAIL;h2B4 |
| Host Species | Rabbit |
| Description | Anti-2B4 antibody(DM69); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | Q9BZW8 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-2B4 antibody(DM69); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human 2B4 protein, mFc-His tagged protein PME100010 can bind Rabbit anti-2B4 monoclonal antibody ( clone: DM69) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human 2B4 (B) were surface stained with Rabbit anti-2B4 monoclonal antibody 1μg/ml ( clone: DM69) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti-2B4 monoclonal antibody ( clone: DM69) on THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Affinity ranking of different Rabbit anti-2B4 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 5. Flow cytometry analysis of antigen binding of rabbit anti-human 2B4 mAb(DME100069).
(A) DME100069 does not bind to CHO-S cells that do not express 2B4.
(B) A clear peak shift of DME100069 was seen compared to the control when incubated with 2B4-expressing THP-1 cells, indicating strong binding of DME100069 to 2B4. Antibodies were incubated at 5 μg/mL.
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