| Clone ID | DM86 |
|---|---|
| Target | |
| Synonyms | CD86; B7-2; B70; CD28LG2; LAB72; MGC34413 |
| Host Species | Rabbit |
| Description | Anti-B7-2 antibody(DM86); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P42081 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells; and it is the ligand for two proteins at the cell surface of T cells; CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-B7-2 antibody(DM86); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-2 protein, mFc-His tagged protein PME100034 can bind Rabbit anti-B7-2 monoclonal antibody (clone: DM86) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human B7-2 (B) were surface stained with Rabbit anti-B7-2 monoclonal antibody 1μg/ml (clone: DM86) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry analysis of antigen binding of rabbit anti-human B7-2 mAb(DME100086).
(A) DME100086 does not bind to 293T cells that do not express B7-2.
(B) A clear peak shift of DME100086 was seen compared to the control when incubated with B7-2-expressing Daudi cells, indicating strong binding of DME100086 to B7-2. Antibodies were incubated at 5 μg/mL
Figure 4. Flow cytometry analysis of antigen binding of rabbit anti-human B7-2 mAb(DME100086).
(A) DME100086 does not bind to 293T cells that do not express B7-2.
(B) A clear peak shift of DME100086 was seen compared to the control when incubated with B7-2-expressing Raji cells, indicating strong binding of DME100086 to B7-2. Antibodies were incubated at 5 μg/mL.
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