| Clone ID | 3G2 |
|---|---|
| Target | |
| Synonyms | BT3.2;BTF4;BTN3.2;CD277 |
| Host Species | Rabbit |
| Description | Anti-BTN3A2 antibody(3G2); IgG1 Chimeric mAb |
| Delivery | In Stock |
| Uniprot ID | P78410 |
| IgG type | Rabbit/Human Fc chimeric IgG1 |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | Flow Cyt |
| Recommended Dilutions | Flow Cyt 1/100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene encodes a member of the immunoglobulin superfamily, which resides in the juxta-telomeric region of the major histocompatability class 1 locus and is clustered with the other family members on chromosome 6. The encoded protein may be involved in the adaptive immune response. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] |
| Usage | Research use only；All DIMA recombinant antibodies are genuinely generated by DIMA Biotech. They are all under patent application. Any protein sequencing or reverse engineering attempt is prohibited. We are actively scrutinizing all patent application to ensure no IP infringement. |
| Conjugate | Unconjugated |
Anti-BTN3A2 antibody(3G2); IgG1 Chimeric mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Flow cytometry analysis with Anti-BTN3A2 (3G2) mAb on Expi293 cells transfected with human BTN3A2 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2. Flow cytometry analysis of antigen binding of anti-human BTN3A2 mAb(DMC101034).
(A) DMC101034 does not bind to MCF-7 cells that do not express BTN3A2.
(B) A clear peak shift of DMC101034 was seen compared to the control when incubated with BTN3A2-expressing 8226 cells, indicating strong binding of DMC101034 to BTN3A2. Antibodies were incubated at 5 μg/mL.
Figure 3. Flow cytometry analysis of antigen binding of anti-human BTN3A2 mAb(DMC101034).
(A) DMC101034 does not bind to MCF-7 cells that do not express BTN3A2.
(B) A clear peak shift of DMC101034 was seen compared to the control when incubated with BTN3A2-expressing Raji cells, indicating strong binding of DMC101034 to BTN3A2. Antibodies were incubated at 5 μg/mL.
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