| Clone ID | DM33 |
|---|---|
| Target | |
| Synonyms | IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; CD123 antigen; CD123; hIL3Ra; hIL-3Ra; MGC34174; IL-3 R alpha |
| Host Species | Rabbit |
| Description | Anti-CD123 antibody(DM33); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P26951 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | Interleukin 3 receptor alpha (low affinity) (IL3RA); also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-?? |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-CD123 antibody(DM33); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD123(right) were surface stained with Rabbit anti-CD123 monoclonal antibody 1μg/ml ( clone: DM33) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 2. Flow cytometry analysis of antigen binding of rabbit anti-human CD123 mAb(DME100032).
(A) DME100032 does not bind to CHO-S cells that do not express CD123.
(B) A clear peak shift of DME100032 was seen compared to the control when incubated with CD123-expressing THP-1 cells, indicating strong binding of DME100032 to CD123. Antibodies were incubated at 5 μg/mL.
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