| Clone ID | DMC213 |
|---|---|
| Target | |
| Synonyms | CD24A |
| Host Species | Rabbit |
| Description | Anti-CD24 antibody(DMC213); IgG1 Chimeric mAb |
| Delivery | In Stock |
| Uniprot ID | P25063 |
| IgG type | Rabbit/Human Fc chimeric IgG1 |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt; WB; IHC |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100; WB 1:1000; IHC 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies; but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1; 15; 20; and Y. Alternative splicing results in multiple transcript variants. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-CD24 antibody(DMC213); IgG1 Chimeric mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Flow cytometry analysis with Anti-CD24 (DMC213) on Expi293 cells transfected with human CD24 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2.Anti-CD24 antibody (SKU# DMC100213) at 1/1000 dilution
Lane : Huh7, whole cell lysate
Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 61 kDa
Observed band size: 25，135 kDa
Figure 3. A. DMC100213 at 10µg/ml staining CD24 in K562-CD24 cells by IHC (SKU# DMC100213);B. DMC100213 at 10µg/ml staining CD24 in human tonsil tissue by IHC (SKU# DMC100213)
Figure 4. Flow cytometry analysis of antigen binding of anti-human CD24 mAb(DMC100213).
(A) DMC100213 does not bind to Jurkat cells that do not express CD24.
(B) A clear peak shift of DMC100213 was seen compared to the control when incubated with CD24-expressing MCF-7 cells, indicating strong binding of DMC100213 to CD24. Antibodies were incubated at 10 μg/mL.
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