| Clone ID | DM63 |
|---|---|
| Target | |
| Synonyms | CD28; Tp44 |
| Host Species | Rabbit |
| Description | Anti-CD28 antibody(DM63); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P10747 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is essential for T-cell proliferation and survival; cytokine production; and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-CD28 antibody(DM63); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD28 protein, mFc-His tagged protein PME100014 can bind Rabbit anti-CD28 monoclonal antibody ( clone: DM63) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human CD28 (B) were surface stained with Rabbit anti-CD28 monoclonal antibody 1μg/ml ( clone: DM63) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti-CD28 monoclonal antibody ( clone: DM63) on Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Affinity ranking of different Rabbit anti- CD28 mAb clones by titration of different concentration onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 5. Flow cytometry analysis of antigen binding of rabbit anti-human CD28 mAb(DME100063).
(A) DME100063 does not bind to 293T cells that do not express CD28.
(B) A clear peak shift of DME100063 was seen compared to the control when incubated with CD28-expressing Jurkat cells, indicating strong binding of DME100063 to CD28. Antibodies were incubated at 2 μg/mL.
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