| Clone ID | DM28 |
|---|---|
| Target | |
| Synonyms | T10; cADPr hydrolase 1 |
| Host Species | Rabbit |
| Description | Anti-CD38 antibody(DM28); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P28907 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; IHC; WB; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | CD antigen CD38 is also known as ADP-ribosyl cyclase 1; which belongs to the ADP-ribosyl cyclase family. CD38 is expressed at high levels in pancreas; liver; kidney; brain; testis; ovary; placenta; malignant lymphoma and neuroblastoma. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD to ADP-ribose. These reaction products are essential for the regulation of intracellular Ca2 . The loss of CD38 function is associated with impaired immune responses; metabolic disturbances; and behavioral modifications. The CD38 protein is a marker of cell activation. It has been connected to HIV infection; leukemias; myelomas; solid tumors; type II diabetes mellitus and bone metabolism. CD38 has been used as a prognostic marker in leukemia. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-CD38 antibody(DM28); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD38 (right) were surface stained with Rabbit anti-CD38 monoclonal antibody 1μg/ml ( clone: DM28) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 2. Flow cytometry analysis of antigen binding of rabbit anti-human CD38 mAb (DME100027). (A) DME100027 does not bind to 293T cells that do not express CD38. (B) A clear peak shift of DME100027 was seen compared to the control when incubated with CD38-expressing H929 cells, indicating strong binding of DME100027 to CD38. Antibodies were incubated at 5 µg/mL.
Figure 3. A. DME100027 at 5µg/ml staining CD38 in RPMI-8226 MM xenografts in NSG mice by IHC (SKU# DME100027);
B: DME100027at 5µg/ml staining CD38 in human tonsil tissue by IHC (SKU# DME100027);
Figure 4. Western blot analysis of CD38 protein using Anti-CD38 antibody (Cat. DME100027) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H L) at 1:5000 dilution.
1. MM.1S cell lysate (native CD38 protein)
2. H929 cell lysate (native CD38 protein)
Figure 5. A. DME100027 at 10µg/ml staining CD38 in M-NSG Daudi DiSliceX™ SlideSet section by IHC (SKU# DME100027);B. DME100027 at 10µg/ml staining CD38 in human tonsil tissue by IHC (SKU# DME100027).
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