| Clone ID | 7E1 |
|---|---|
| Target | |
| Synonyms | B220; CD45; CD45R; GP180; L-CA; LCA; LY5; T200 |
| Host Species | Rabbit |
| Description | Anti-CD45 antibody(7E1), IgG1 Chimeric mAb |
| Delivery | In Stock |
| Uniprot ID | P08575 |
| IgG type | Rabbit/Human Fc chimeric IgG1 |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | IHC |
| Recommended Dilutions | IHC 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth; differentiation; mitosis; and oncogenic transformation. This PTP contains an extracellular domain; a single transmembrane segment and two tandem intracytoplasmic catalytic domains; and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes; or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases; and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene; which encode distinct isoforms; have been reported. |
| Usage | Research use only；All DIMA recombinant antibodies are genuinely generated by DIMA Biotech. They are all under patent application. Any protein sequencing or reverse engineering attempt is prohibited. We are actively scrutinizing all patent application to ensure no IP infringement. |
| Conjugate | Unconjugated |
Anti-CD45 antibody(7E1), IgG1 Chimeric mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. A. DMC100725 at 10µg/ml staining CD45 in Hut78 cells by IHC (SKU# DMC100725);B. DMC100725 at 10µg/ml staining CD45 in human tonsil tissue by IHC (SKU# DMC100725)
Figure 2. Flow cytometry analysis of antigen binding of anti-human CD45 mAb(DMC100725).
(A) DMC100725 does not bind to CHO-S cells that do not express CD45.
(B) A clear peak shift of DMC100725 was seen compared to the control when incubated with CD45-expressing THP-1 cells, indicating strong binding of DMC100725 to CD45. Antibodies were incubated at 5 μg/mL.
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