Anti-CD48 antibody(DM44), Rabbit mAb

Name: Anti-CD48 antibody(DM44), Rabbit mAb - DIMA Biotechnology
SKU: DME100043
Price: 109900100438005006336.00 USD
Availability: InStock

Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00

SKU: DME100043 Category: Monoclonal antibodies

Datasheet

MSDS

Product Data

Clone ID	DM44
Target	CD48
Synonyms	CD48; BCM1; SLAMF2; BLAST; BLAST1; MEM-102; TCT.1; BCM-1; SLAMF-2; BLAST-1
Host Species	Rabbit
Description	Anti-CD48 antibody(DM44); Rabbit mAb
Delivery	In Stock
Uniprot ID	P09326
IgG type	Rabbit IgG
Clonality	Monoclonal
Reactivity	Human
Applications	ELISA; Flow Cyt; WB
Recommended Dilutions	ELISA 1:5000-10000; Flow Cyt 1:100; WB 1:1000
Purification	Purified from cell culture supernatant by affinity chromatography
Formulation & Reconstitution	Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Storage & Shipping	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Background	This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells; dendritic cells and endothelial cells; and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain; however; but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.
Usage	Research use only
Conjugate	Unconjugated

Images

DME100043-CD48-Fig.1-FC-1.jpg
Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD48 (right) were surface stained with Rabbit anti-CD48 monoclonal antibody 1μg/ml ( clone: DM44) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD48 monoclonal antibody ( clone: DM44) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Figure 4. Flow cytometry analysis of antigen binding of rabbit anti-human CD48 mAb(DME100043).

(A) DME100043 does not bind to 293T cells that do not express CD48.
(B) A clear peak shift of DME100043 was seen compared to the control when incubated with CD48-expressing Raji cells, indicating strong binding of DME100043 to CD48. Antibodies were incubated at 2 μg/mL.

Figure 5. Anti-CD48 antibody (SKU# DME100043) at 1/1000 dilution