| Clone ID | DM204 |
|---|---|
| Target | |
| Synonyms | CD73;NT5E;5'-Nucleotidase;5'-NT;NT5;NTE |
| Host Species | Rabbit |
| Description | Anti-CD73 antibody(DM204); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P21589 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq; Mar 2011] |
| Usage | Research use only；All DIMA recombinant antibodies are genuinely generated by DIMA Biotech. They are all under patent application. Any protein sequencing or reverse engineering attempt is prohibited. We are actively scrutinizing all patent application to ensure no IP infringement. |
| Conjugate | Unconjugated |
Anti-CD73 antibody(DM204); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Flow cytometry analysis with Anti-CD73 (DM204) on Expi293 cells transfected with human CD73 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2. Flow cytometry analysis of antigen binding of Rabbit anti-human CD73 mAb(DME100204).
(A) DME100204 does not bind to Jurkat cells that do not express CD73.
(B) A clear peak shift of DME100204 was seen compared to the control when incubated with CD73-expressing HT55 cells, indicating strong binding of DME100204 to CD73. Antibodies were incubated at 10 μg/mL.
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