| Clone ID | 52A11 |
|---|---|
| Target | |
| Synonyms | AGM6;B29;IGB |
| Host Species | Rabbit |
| Description | Anti-CD79B antibody(52A11); IgG1 Chimeric mAb |
| Delivery | In Stock |
| Uniprot ID | P40259 |
| IgG type | Rabbit/Human Fc chimeric IgG1 |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | Flow Cyt; WB |
| Recommended Dilutions | Flow Cyt 1:100; WB 1:1000 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-CD79B antibody(52A11); IgG1 Chimeric mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Flow cytometry analysis with Anti-CD79B (52A11) mAb on Expi293 cells transfected with human CD79B (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2. Flow cytometry analysis of antigen binding of anti-human CD79B mAb(DMC101033).
(A) DMC101033 does not bind to 293T cells that do not express CD79B.
(B) A clear peak shift of DMC101033 was seen compared to the control when incubated with CD79B-expressing Raji cells, indicating strong binding of DMC101033 to CD79B. Antibodies were incubated at 5 μg/mL.
Figure 3.Anti-CD79B antibody (SKU# DMC101033) at 1/1000 dilution
Lane : RAJI (human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 26 kDa
Observed band size: 24 kDa
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