| Clone ID | DM203 |
|---|---|
| Target | |
| Synonyms | HBA71; MIC2; MIC2X; MIC2Y; MSK5X |
| Host Species | Rabbit |
| Description | Anti-CD99 antibody(DM203); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P14209 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt; IHC |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100; IHC 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration; T-cell adhesion; ganglioside GM1 and transmembrane protein transport; and T-cell death by a caspase-independent pathway. In addition; the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq; Mar 2016] |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-CD99 antibody(DM203); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. CD99 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CD99 (DM203) on Expi293 cells transfected with human CD99 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).
Figure 2. A. DME100203 at 10µg/ml staining CD99 in RPMI-8226 MM xenografts in NSG mice by IHC (SKU# DME100203);B: DME100203 at 10µg/ml staining CD99 in in human tonsil tissue by IHC (SKU# DME100203).
Figure 3. Flow cytometry analysis of antigen binding of rabbit anti-human CD99 mAb(DME100203).
(A) DME100203 does not bind to CHO-S cells that do not express CD99.
(B) A clear peak shift of DME100203 was seen compared to the control when incubated with CD99-expressing 8226 cells, indicating strong binding of DME100203 to CD99. Antibodies were incubated at 5 μg/mL.
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