| Clone ID | 5F5 |
|---|---|
| Target | |
| Synonyms | CDH16;HPT-1;HPT1 |
| Host Species | Rabbit |
| Description | Anti-CDH17 antibody(5F5), IgG1 Chimeric mAb |
| Delivery | In Stock |
| Uniprot ID | Q12864 |
| IgG type | Rabbit/Human Fc chimeric IgG1 |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | WB; Flow Cyt |
| Recommended Dilutions | WB 1:1000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009] |
| Usage | Research use only；All DIMA recombinant antibodies are genuinely generated by DIMA Biotech. They are all under patent application. Any protein sequencing or reverse engineering attempt is prohibited. We are actively scrutinizing all patent application to ensure no IP infringement. |
| Conjugate | Unconjugated |
Anti-CDH17 antibody(5F5), IgG1 Chimeric mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1.Anti-CDH17 antibody (SKU# DMC100637) at 1/1000 dilution
Lane 1: AGS, whole cell lysate
Lane 2: HT55, whole cell lysate
Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 92 kDa
Observed band size: 120 kDa
Figure 2. Flow cytometry analysis of antigen binding of anti-human CDH17 mAb(DMC100637).
(A) DMC100637 does not bind to 293T cells that do not express CDH17
(B) A clear peak shift of DMC100637 was seen compared to the control when incubated with CDH17 -expressing HT55 cells, indicating strong binding of DMC100637 to CDH17.
Antibodies were incubated at 2ug/mL.
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