| Clone ID | DMC687 |
|---|---|
| Target | |
| Synonyms | CAR; CAR4:6; HCAR |
| Host Species | Rabbit |
| Description | Anti-CXADR antibody(DMC687); IgG1 Chimeric mAb |
| Delivery | In Stock |
| Uniprot ID | P78310 |
| IgG type | Rabbit/Human Fc chimeric IgG1 |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | Flow Cyt; WB; IHC |
| Recommended Dilutions | Flow Cyt 1:100; WB 1:1000; IHC 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15; 18; and 21. [provided by RefSeq; May 2011] |
| Usage | Research use only；All DIMA recombinant antibodies are genuinely generated by DIMA Biotech. They are all under patent application. Any protein sequencing or reverse engineering attempt is prohibited. We are actively scrutinizing all patent application to ensure no IP infringement. |
| Conjugate | Unconjugated |
Anti-CXADR antibody(DMC687), IgG1 Chimeric mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. CXADR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CXADR (DMC687) on Expi293 cells transfected with human CXADR (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).
Figure 2. Flow cytometry analysis of antigen binding of anti-human CXADR mAb(DMC100687).
(A) DMC100687 does not bind to K562 cells that do not express CXADR.
(B) A clear peak shift of DMC100687 was seen compared to the control when incubated with CXADR-expressing Huh7 cells, indicating strong binding of DMC100687 to CXADR. Antibodies were incubated at 5 μg/mL.
Figure 3.Anti-CXADR antibody (SKU# DMC100687) at 1/1000 dilution
Lane : Huh7, whole cell lysate
Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 40 kDa
Observed band size: 50 kDa
Figure 4. A. DMC100687 at 10µg/ml staining CXADR in Huh7 xenografts in nude mice by IHC (SKU# DMC100687);B. DMC100687 at 10µg/ml staining CXADR in human tonsil tissue by IHC (SKU# DMC100687)
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