| Common Name | Fab C225,IMC-225 |
|---|---|
| Synonyms | EGFR;ERBB;ERBB1;HER1;PIG61;mENA |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000 |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Host Species | Chimeric |
| IgG type | IgG1 |
| Reactivity | Human |
| Target | |
| Uniprot ID | P00533 |
| Description | Anti-EGFR (Cetuximab biosimilar) mAb |
| Delivery | In Stock |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| Usage | Research use only；All DIMA recombinant antibodies are genuinely generated by DIMA Biotech. They are all under patent application. Any protein sequencing or reverse engineering attempt is prohibited. We are actively scrutinizing all patent application to ensure no IP infringement. |
| Conjugate | Unconjugated |
Anti-EGFR (Cetuximab biosimilar) mAb
Price: 50μg $82.00 ; 100 μg $162.00
Product Data
Images
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human EGFR, His tagged protein PME100099 can bind Anti-EGFR Neutralizing antibody in a linear range of 0.64-80 ng/ml.
Figure 2. EGFR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EGFR (Cetuximab) on Expi293 cells transfected with human EGFR(Blue histogram) or Expi293 transfected with irrelevant protein(Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein(Orange histogram)
Figure 3. Flow cytometry analysis of antigen binding of anti-human EGFR mAb(BME100034).
(A) A clear peak shift of BME100034 was seen compared to the control when incubated with EGFR-expressing Hela cells, indicating strong binding of BME100034 to EGFR. Antibodies were incubated at 2 μg/mL.
Figure 4. Flow cytometry analysis of antigen binding of anti-human EGFR mAb(BME100034).
(A) BME100034 does not bind to Jurkat cells that do not express EGFR.
(B) A clear peak shift of BME100034 was seen compared to the control when incubated with EGFR-expressing A431 cells, indicating strong binding of BME100034 to EGFR. Antibodies were incubated at 5 μg/mL.
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