| Clone ID | 1B8 |
|---|---|
| Target | |
| Synonyms | Glypican 1;PC1 |
| Host Species | Rabbit |
| Description | Anti-GPC1 antibody(1B8); IgG1 Chimeric mAb |
| Delivery | In Stock |
| Uniprot ID | P35052 |
| IgG type | Rabbit/Human Fc chimeric IgG1 |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | Flow Cyt |
| Recommended Dilutions | Flow Cyt 1/100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008] |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-GPC1 antibody(1B8); IgG1 Chimeric mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Flow cytometry analysis with Anti-GPC1 (1B8) mAb on Expi293 cells transfected with human GPC1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2. Flow cytometry analysis of antigen binding of anti-human GPC1 mAb(DMC101004).
(A) DMC101004 does not bind to Jurkat cells that do not express GPC1.
(B) A clear peak shift of DMC101004 was seen compared to the control when incubated with GPC1-expressing 8226 cells, indicating strong binding of DMC101004 to GPC1. Antibodies were incubated at 10 μg/mL.
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