| Clone ID | 7F12 |
|---|---|
| Target | |
| Synonyms | MIC-A; PERB11.1 |
| Host Species | Rabbit |
| Description | Anti-MICA antibody(7F12), IgG1 Chimeric mAb |
| Delivery | In Stock |
| Uniprot ID | Q29983 |
| IgG type | Rabbit/Human Fc chimeric IgG1 |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | WB; Flow Cyt |
| Recommended Dilutions | WB 1:1000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-MICA antibody(7F12), IgG1 Chimeric mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1.Anti-MICA antibody (SKU# DMC100608) at 1/1000 dilution
Lane : HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate
Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution
band size: 43 kDa
Figure 2. Flow cytometry analysis of antigen binding of anti-human MICA mAb(DMC100608).
(A) DMC100608 does bind to PC3 cells that weakly express MICA
(B) A clear peak shift of DMC100608 was seen compared to the control when incubated with MICA-expressing Hela cells, indicating strong binding of DMC100608 to MICA.
Antibodies were incubated at 10 ug/mL.
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