| Clone ID | DM73 |
|---|---|
| Target | |
| Synonyms | MSLN; Mesothelin; MPF |
| Host Species | Rabbit |
| Description | Anti-mesothelin antibody(DM73); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | Q13421 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt; WB |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100; WB 1:1000 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene encodes a preproprotein that is proteolytically processed to generate two protein products; megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas; ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants; at least one of which encodes an isoform that is proteolytically processed. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-mesothelin antibody(DM73), Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human MSLN protein, mFc-His tagged protein PME100031 can bind Rabbit anti-MSLN monoclonal antibody ( clone: DM73) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human mesothelin (B) were surface stained with Rabbit anti-MSLN monoclonal antibody 1μg/ml ( clone: DM73) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody ( clone: DM73) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Affinity ranking of different Rabbit anti-MSLN mAb clones by titration of different concentration onto Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 5. Flow cytometry analysis of antigen binding of rabbit anti-human Mesothelin mAb(DME100073).
(A) DME100073 does not bind to 293T cells that do not express Mesothelin.
(B) A clear peak shift of DME100073 was seen compared to the control when incubated with Mesothelin-expressing Hela cells, indicating strong binding of DME100073 to Mesothelin. Antibodies were incubated at 2 μg/mL.
Figure 6. Anti-MSLN antibody (SKU# DME100073) at 1/1000 dilution
Lane : HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate
Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 69 kDa
Observed band size: 50 kDa
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