| Clone ID | DM159 |
|---|---|
| Target | |
| Synonyms | NTB-A;SLAMF6;Ly108;NK-T-B-antigen;CD352;KALI |
| Host Species | Rabbit |
| Description | Anti-NTB-A antibody(DM159); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | Q96DU3 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt; WB; IHC |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100; WB 1:1000; IHC 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a type I transmembrane protein; belonging to the CD2 subfamily of the immunoglobulin superfamily. This encoded protein is expressed on Natural killer (NK); T; and B lymphocytes. It undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases (SHPs). It functions as a coreceptor in the process of NK cell activation. It can also mediate inhibitory signals in NK cells from X-linked lymphoproliferative patients. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-NTB-A antibody(DM159); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data
Images
Figure 1. Flow cytometry analysis with Anti-NTB-A (DM159) on Expi293 cells transfected with human NTB-A (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2. Flow cytometry analysis of antigen binding of rabbit anti-human NTB-A mAb(DME100159).
(A) DME100159 does not bind to 293T cells that do not express NTB-A.
(B) A clear peak shift of DME100159 was seen compared to the control when incubated with NTB-A-expressing Raji cells, indicating strong binding of DME100159 to NTB-A. Antibodies were incubated at 5 μg/mL.
Figure 3. Flow cytometry analysis of antigen binding of rabbit anti-human NTB-A mAb(DME100159).
(A) DME100159 does not bind to 293T cells that do not express NTB-A.
(B) A clear peak shift of DME100159 was seen compared to the control when incubated with NTB-A-expressing Jurkat cells, indicating strong binding of DME100159 to NTB-A. Antibodies were incubated at 5 μg/mL.
Figure 4.Anti-NTB-A antibody (SKU# DME100159) at 1/1000 dilution
Lane : RAJI(human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 37 kDa
Observed band size: 60 kDa
Figure 5. DME100159 at 10µg/ml staining NTB-A in Raji cells by IHC (SKU# DME100159)
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