| Common Name | AGX101 |
|---|---|
| Synonyms | M3S1;TAAL6 |
| Applications | Flow Cyt |
| Recommended Dilutions | Flow Cyt 1:100 |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Host Species | Humanized |
| IgG type | IgG1 |
| Reactivity | Human |
| Target | |
| Uniprot ID | P30408 |
| Description | Anti-TM4SF1 (AGX101 biosimilar) mAb |
| Delivery | In Stock |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| Usage | Research use only；All DIMA recombinant antibodies are genuinely generated by DIMA Biotech. They are all under patent application. Any protein sequencing or reverse engineering attempt is prohibited. We are actively scrutinizing all patent application to ensure no IP infringement. |
| Conjugate | Unconjugated |
Anti-TM4SF1 (AGX101 biosimilar) mAb
Price: 50μg $82.00 ; 100 μg $162.00
Product Data
Images
Figure 1. Flow cytometry analysis with 1 μg/mL Anti-TM4SF1 (AGX101 biosimilar) mAb (BME100159) on Expi293 cells transfected with Human TM4SF1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2. ELISA plates were pre-coated with 0.5μg/per well purified human TM4SF1 full length membrane nanoparticles (Cat# FLP100021). Serial diluted Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# BME100159) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-TM4SF1 (AGX101 biosimilar) mAb binding with TM4SF1 MNPs (Cat# FLP100021) is 4.174ng/ml.
Figure 3. ELISA plates were pre-coated with Flag Tag TM4SF1-Nanodisc (Cat# FLP100022, 0.2μg/per well). Serial diluted Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# BME100159) were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# BME100159) binding with TM4SF1-Nanodisc (Cat# FLP100022) is 15.97ng/ml.
Figure 4. FACS analysis of TM4SF1 MNPs (Cat# FLP100021)
A. TM4SF1 MNP (Cat# FLP100021) were stained only with Goat anti-human 2nd antibody.
B. Negative control MNP samples were stained with Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# BME100159) at 2μg/mL, followed by Goat anti-human IgG 488 secondary antibody.
C. TM4SF1 MNP (Cat# FLP100021) samples were stained with anti-CCR8 antibody (an irrelevant antibody) at 2μg/mL, followed by Goat anti-human 2nd antibody.
D. TM4SF1 MNP (Cat# FLP100021) samples were stained with Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# BME100159) at 2μg/mL, followed by Goat anti-human 2nd antibody.
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