| Common Name | AGX101 |
|---|---|
| Synonyms | M3S1;TAAL6 |
| Applications | Flow Cyt |
| Recommended Dilutions | Flow Cyt 1:100 |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Host Species | Humanized |
| IgG type | IgG1 |
| Reactivity | Human |
| Target | |
| Uniprot ID | P30408 |
| Description | Anti-TM4SF1 (AGX101 biosimilar) mAb |
| Delivery | In Stock |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-TM4SF1 (AGX101 biosimilar) mAb
Price: 50μg $82.00 ; 100 μg $162.00
Product Data
Images
Figure 1. Flow cytometry analysis with 1 μg/mL Anti-TM4SF1 (AGX101 biosimilar) mAb (BME100159) on Expi293 cells transfected with Human TM4SF1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2. ELISA plates were pre-coated with 0.5μg/per well purified human TM4SF1 full length membrane nanoparticles (Cat# FLP100021). Serial diluted Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# BME100159) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-TM4SF1 (AGX101 biosimilar) mAb binding with TM4SF1 MNPs (Cat# FLP100021) is 4.174ng/ml.
Figure 3. ELISA plates were pre-coated with Flag Tag TM4SF1-Nanodisc (Cat# FLP100022, 0.2μg/per well). Serial diluted Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# BME100159) were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# BME100159) binding with TM4SF1-Nanodisc (Cat# FLP100022) is 15.97ng/ml.
Figure 4. FACS analysis of TM4SF1 MNPs (Cat# FLP100021)
A. TM4SF1 MNP (Cat# FLP100021) were stained only with Goat anti-human 2nd antibody.
B. Negative control MNP samples were stained with Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# BME100159) at 2μg/mL, followed by Goat anti-human IgG 488 secondary antibody.
C. TM4SF1 MNP (Cat# FLP100021) samples were stained with anti-CCR8 antibody (an irrelevant antibody) at 2μg/mL, followed by Goat anti-human 2nd antibody.
D. TM4SF1 MNP (Cat# FLP100021) samples were stained with Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# BME100159) at 2μg/mL, followed by Goat anti-human 2nd antibody.
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