| Target | |
|---|---|
| Description | Monoclonal Cell Line Derived from 293T Cells, Engineered for Stable Expression of Human CD33 Using Lentiviral Technology |
| Host Cells | 293T |
| Uniprot ID | P20138 |
| Applications | FACS Data |
| Growth media | DMEM+10% FBS+1% P.S+Gln+2 ug/mL Puromycin |
| Package | 5E6 Cells/mL |
| Host Species | Human |
| Suggested Control | SKU: BME100015 |
| Warranty and Disclaimer | 1. Please inspect cells upon receipt and report any issues promptly. 2. We offer one-time replacements for issues reported within a week of receipt. 3. User-induced issues are not eligible for free replacements. 4. We do not accept liability for damages resulting from cell use, storage, or loss. 5. Feedback received more than one month after receipt will not be processed. |
| Storage & Shipping | Cells are shipped using dry ice and require liquid nitrogen storage for long term preservation. |
| Synonyms | CD33;SIGLEC3;gp67 |
| Background | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins; two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6:SHP-1 and PTPN11:SHP-2. In turn; these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase:PI3K. |
| Usage | For research use only. |
Hu_CD33 293T Cell Line
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Images
Figure 1. Flow cytometry analysis of human CD33 overexpression using Hu_CD33 293T Cell Line (Cat. No. CEL100025) and Anti-CD33 (gemtuzumab biosimilar) mAb (Cat. No. BME100015)