| Tag | C-Flag Tag |
|---|---|
| Target | |
| Synonyms | CANN6; CB-R; CNR1; CB1A; CB1K5; CB1R; CNR |
| Description | Human CB1 full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | P21554 |
| Expression Host | HEK293 |
| Protein Families | GPCR |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| Molecular Weight | The human full length CB1 Protein has a MW of 52.7 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human CB1 full length protein-synthetic nanodisc
Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00
Product Data
Images
Figure1. Elisa plates were pre-coated with Flag Tag CB1-Nanodisc (0.2μg/per well). Serial diluted anti-CB1 monoclonal antibody (DME100144) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CB1 monoclonal antibody binding with CB1-Nanodisc is 41.62ng/ml.
Figure2. Human CB1-Nanodisc, Flag Tag on SDS-PAGE
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