| Target | |
|---|---|
| Synonyms | CD27;TNFRSF7;S152;T14;Tp55 |
| Description | Recombinant human CD27 protein with C-terminal mouse Fc and 6×His tag |
| Delivery | In Stock |
| Uniprot ID | P26842 |
| Expression Host | HEK293 |
| Tag | C-Mouse Fc and 6×His Tag |
| Molecular Characterization | CD27(Ala20-Arg191) mFc(Pro99-Lys330) 6×His |
| Molecular Weight | The protein has a predicted molecular mass of 65-72 kDa after removal of the signal peptide. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human CD27 Protein, mFc-His Tag
Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00
Product Data
Images
Figure 1. Human CD27 Protein, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD27, mFc-His tagged protein (PME100013) can bind Anti-CD27 Neutralizing antibody BME100018 in a linear range of 0.64-16.0 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD27, mFc-His tagged protein (PME100013) can bind Human CD70,hFc-His tagged protein PME100486 in a linear range of 3.2-80 ng/ml.
Figure 4. Flow cytometry analysis with 15 μg/mL Human CD27 Protein, mFc-His tag (PME100013) on Expi293 cells transfected with human CD70 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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