| Target | |
|---|---|
| Synonyms | CD28;Tp44 |
| Description | Recombinant human CD28 protein with C-terminal mouse Fc and 6×His tag |
| Delivery | In Stock |
| Uniprot ID | P10747 |
| Expression Host | HEK293 |
| Tag | C-Mouse Fc and 6×His Tag |
| Molecular Characterization | CD28(Asn19-Pro152) mFc(Pro99-Lys330) 6×His |
| Molecular Weight | The protein has a predicted molecular mass of 68-72 kDa after removal of the signal peptide. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human CD28 Protein, mFc-His Tag
Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00
Product Data
Images
Figure 1. Human CD28 Protein, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-1, hFc tagged protein PME100473 can bind Human CD28, mFc-His tagged protein (PME100014) in a linear range of 125-4000 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-2, hFc tagged protein PME100474 can bind Human CD28, mFc-His tagged protein (PME100014) in a linear range of 62.5-4000 ng/ml.
Figure 4. HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28, mFc-His tagged protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
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