| Target | |
|---|---|
| Synonyms | CD40LG;CD154;CD40L;HIGM1;IGM;IMD3;T-BAM;TNFSF5;TRAP;gp39 |
| Description | Recombinant human CD40 Ligand Protein with N-terminal Human Fc tag |
| Delivery | In Stock |
| Uniprot ID | P29965 |
| Expression Host | HEK293 |
| Tag | N-Human Fc Tag |
| Molecular Characterization | hFc(Glu99-Ala330) CD40 Ligand(Met113-Leu261) |
| Molecular Weight | The protein has a predicted molecular mass of 42.3 kDa after removal of the signal peptide.The apparent molecular mass of hFc-CD40 Ligand is approximately 40-53 kDa due to glycosylation. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human CD40 Ligand Protein, hFc Tag
Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00
Product Data
Images
Figure 1. Human CD40 Ligand Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD40 Ligand,hFc tagged protein (PME100192) can bind Human CD40, mFc-His tagged protein PME100015 in a linear range of 0.51-320 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD40L, hFc tagged protein (PME100192) can bind Anti-CD40L Neutralizing antibody BME100042 in a linear range of 0.64-400 ng/ml.
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