| Tag | C-Flag Tag |
|---|---|
| Target | |
| Synonyms | CD182; CD183; CKR-L2; CMKAR3; GPR9; IP10-R; Mig-R; MigR |
| Description | Human CXCR3 full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | P49682 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Chemokine signaling pathway, Cytokine-cytokine receptor interaction |
| Molecular Weight | The human full length CXCR3 protein has a MW of 40.6 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | A G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human CXCR3 full length protein-synthetic nanodisc
Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00
Product Data
Images
Figure1. Elisa plates were pre-coated with Flag Tag CXCR3-Nanodisc (0.2μg/per well). Serial diluted anti-CXCR3 monoclonal antibody (DME100208) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CXCR3 monoclonal antibody binding with CXCR3-Nanodisc is 15.79ng/ml.
Figure2. WB analysis of Human CXCR3-Nanodisc with anti-Flag monoclonal antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution
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