Target | |
---|---|
Synonyms | ALXDRD |
Description | Recombinant human GFAP(68-377) protein with N-terminal 6×His tag |
Delivery | In Stock |
Uniprot ID | P14136 |
Expression Host | HEK293 |
Tag | N-6×His Tag |
Molecular Characterization | 6×His tag GFAP(Ser68-Ile377) |
Molecular Weight | The protein has a predicted molecular mass of 37.2 kDa after removal of the signal peptide. The apparent molecular mass of His-GFAP(68-377) is approximately 35-55 kDa due to glycosylation. |
Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
Usage | Research use only |
Conjugate | Unconjugated |
Human GFAP (68-377) Protein, His Tag
Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00
Product Data
Images
Figure 1. Human GFAP(68-377) Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human GFAP (68-377) Protein, His Tag (PME100667) can bind Anti-GFAP(68-377) antibody(DM216), Rabbit mAb (DME100216) in a linear range of 0.13–16 ng/mL.
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