| Target | |
|---|---|
| Synonyms | ATAR;CD270;HVEA;HVEM;LIGHTR;TR2 |
| Description | Recombinant Human HVEM Protein with C-terminal 6×His tag |
| Delivery | In Stock |
| Uniprot ID | Q92956 |
| Expression Host | HEK293 |
| Tag | C-6×His Tag |
| Molecular Characterization | HVEM(Leu39-Val202) 6×His tag |
| Molecular Weight | The protein has a predicted molecular mass of 18.2 kDa after removal of the signal peptide.The apparent molecular mass of HVEM-His is approximately 25-55 kDa due to glycosylation. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human HVEM Protein, His Tag
Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00
Product Data
Images
Figure 1. Human HVEM Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human HVEM, His tagged protein (PME100273) can bind Human BTLA,mFc-His tagged protein PME100048 in a linear range of 0.64-80 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human HVEM, His tagged protein (PME100273) can bind Human CD160,hFc tagged protein PME100055 in a linear range of 1.28-160 ng/ml.
Figure 4. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human HVEM, His tagged protein (PME100273) can bind Human LIGHT, hFc tagged protein PME100049 in a linear range of 0.32-40 ng/ml.
Figure 5. Flow cytometry analysis with 15 μg/mL Human HVEM Protein, His tag (PME100273) on Expi293 cells transfected with human BTLA (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 6. Flow cytometry analysis with 15 μg/mL Human HVEM Protein, His tag (PME100273) on Expi293 cells transfected with human LIGHT (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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