| Target | |
|---|---|
| Synonyms | IL-12B;CLMF p40;IL-12 subunit p40;NKSF2 |
| Description | Recombinant human IL12B protein with C-terminal 6×His tag |
| Delivery | In Stock |
| Uniprot ID | P29460 |
| Expression Host | HEK293 |
| Tag | C-6×His Tag |
| Molecular Characterization | IL12B(Ile23-Ser328) 6×His tag |
| Molecular Weight | The protein has a predicted molecular mass of 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL12B-His is approximately 35-55 kDa due to glycosylation. |
| Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008] |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human IL12B Protein, His Tag
Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00
Product Data
Images
Figure 1. Human IL12B Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human IL12B Protein, His Tag (PME101133) can bind Anti-IL12B(ebdarokimab biosimilar) mAb (BME100223) in a linear range of 0.13–16 ng/mL.
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