Tag | C-Flag&Strep Tag |
---|---|
Target | |
Synonyms | ERG-1; ERG1; H-ERG; HERG; HERG1; Kv11.1; LQT2; SQT1 |
Description | Human KCNH2-Strep full length protein-synthetic nanodisc |
Delivery | In Stock |
Uniprot ID | Q12809 |
Expression Host | HEK293 |
Protein Families | Druggable Genome, Ion Channels: Potassium, Transcription Factors, Transmembrane |
Protein Pathways | N/A |
Molecular Weight | The human full length KCNH2-Strep protein has a MW of 126.7 kDa |
Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | A voltage-activated potassium channel belonging to the eag family. It shares sequence similarity with the Drosophila ether-a-go-go (eag) gene. Mutations in this gene can cause long QT syndrome type 2 (LQT2). |
Usage | Research use only |
Conjugate | Unconjugated |
Human KCNH2-Strep full length protein-synthetic nanodisc
Price: 10 μg $810.00 ; 50 μg $3960.00 ; 100 μg $7200.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag KCNH2-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with KCNH2-Strep-nanodisc is 2.328ng/ml.
Figure 2. Human KCNH2-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
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