| Target | |
|---|---|
| Synonyms | CSF-1;MCSF |
| Description | Recombinant Human M-CSF Protein with C-terminal human Fc tag |
| Delivery | In Stock |
| Uniprot ID | P09603 |
| Expression Host | HEK293 |
| Tag | C-Human Fc Tag |
| Molecular Characterization | M-CSF(Glu33-Arg255) hFc(Glu99-Ala330) |
| Molecular Weight | The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide.The apparent molecular mass of M-CSF-hFc is approximately 55-70 kDa due to glycosylation. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011] |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human M-CSF Protein, hFc Tag
Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00
Product Data
Images
Figure 1. Human M-CSF Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CSF1R, His tagged protein PME100067 can bind Human M-CSF, hFc Tagged protein（PME100550） in a linear range of 0.32-200 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human M-CSF Protein, hFc Tag ( PME100550) can bind Anti-M-CSF Neutralizing antibody BME100104 in a linear range of 9.77–156.25 ng/mL.
Figure 4. Flow cytometry analysis with 1 μg/mL Human M-CSF Protein, hFc tag (PME100550) on Expi293 cells transfected with human CSF1R (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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