Synthetic Nanodisc: An Innovative Solution For Multipass Transmembrane Proteins

Discover a breakthrough in membrane protein research and drug development with DIMA Biotech’s Synthetic Nanodiscs. Traditional methods struggle with challenges in obtaining water-soluble and bioactive forms of multi-transmembrane proteins. Our innovative polymer-based Nanodisc platform offers a game-changing solution by excluding membrane scaffold proteins (MSPs), resulting in a cleaner system with fewer interferences for downstream assays. By disassembling cell membranes into nanoscale disc-shaped structures, our technology enables the purification of membrane proteins to high homogeneity under aqueous conditions. Explore how Synthetic Nanodiscs empower the functional characterization of multi-span transmembrane proteins in their active form, revolutionizing the future of membrane protein research and drug development.

Explore Our Nanodisc Collection!

Case Studies

Human SLC7A11 full length protein-synthetic nanodisc (Cat# FLP100048) 12 pass transmembrane protein

page-FLP100048 SLC7A11 Fig.2 SDS PAGE 1

Human SLC7A11-Nanodisc, Flag Tag on SDS-PAGE

elisa-FLP100048 SLC7A11 Fig.1 Elisa 1

Flagged tagged SLC7A11-Nanodisc (FLP100048) can bind anti-Flag mAb and the EC50 is 4.101ng/ml.

SPR-flp100048 slc7a11 sec hplc1

The purity of Human SLC7A11-Nanodisc (FLP100048) is greater than 90% as determined by SEC-HPLC.

Human CLDN18.2 full length protein-synthetic nanodisc (Cat# FLP100014) 4 pass transmembrane protein

page-FLP100014 CLDN18.2 Fig.2 SDS PAGE 1

Human CLDN18.2-Nanodisc, Flag Tag on SDS-PAGE

elisa-FLP100014 CLDN18.2 Fig.1 Elisa 1

Human CLDN18.2-Nanodisc (0.2μg/per well) binds to anti- CLDN18.2 mAb (Cat. No. BME100075) (EC50 593.6ng/ml).

SPR-FLP100014 CLDN18.2 Fig.3 SPR 1

Human CLDN18.2-Nanodisc can bind Anti-CLDN18.2 antibody (BME100075) with an affinity constant of 1.619 nM as determined in a SPR assay.

Human MDR-1 full length protein-synthetic nanodisc (Cat# FLP100029) 12 pass transmembrane protein

Human MDR-1-Nanodisc, Flag Tag on SDS-PAGE

Human MDR-1-Nanodisc, Flag Tag on SDS-PAGE

ELISA assay to evaluate MDR-1-Nanodisc

MDR-1-Nanodisc (FLP100029) can bind anti-Flag monoclonal antibody and the EC50 is 6.883ng/ml.

Human GPRC5D full length protein-synthetic nanodisc (Cat# FLP100011) 7 pass transmembrane protein

Human GPRC5D-Nanodisc, Flag Tag on SDS-PAGE

Human GPRC5D-Nanodisc, Flag Tag on SDS-PAGE

GPRC5D-Nanodisc (FLP100011) can bind anti-GPRC5D mAb (DME100090) and the EC50 is 32.86ng/ml.

Human CD63 full length protein-synthetic nanodisc (Cat#: FLP100030)

High Activity

ELISA

pages-Full length Membrane Proteins High Activity High Stability 4

High Stability

Lyophilization test

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Accelerated Test (Lyophilized Powder)

pages-Full length Membrane Proteins High Activity High Stability 6

Synthetic Nanodiscs

Nanodisc Platform

The diagram of nanodisc

Advantages:
・Full length membrane proteins with high purity
・High solubility in aqueous solutions
・High stability, lyophilized powder stable at room temperature 
・Proteins are in a native membrane environment and remain biologically active
・No detergent, useful for cell-based assays
・No MSP backbone proteins

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Applications:
・ELISA
・SPR affinity analysis
・Phage display screening
・Immunization
・Cell based assays
・Protein crystal structure analysis

Nanodiscs Products & Service

Off-the-shelf Nanodisc Proteins

The structure of MSP-Nanodisc (left) and Synthetic Nanodisc (right)

Blog

Synthetic Nanodisc, A Promising Solution for “Undruggable” Membrane Proteins

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    Synthetic Nanodiscs Products

    elisa-FLP100108 GPR20 Fig.1 Elisa 1

    Full Length Transmembrane Proteins

    Human GPR20 full length protein-synthetic nanodisc

    SKU:  FLP100108     Target:  GPR20

    Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00

    elisa-FLP100107 ADGRG2 Fig.1 Elisa 1

    Full Length Transmembrane Proteins

    Human ADGRG2 full length protein-synthetic nanodisc

    SKU:  FLP100107     Target:  ADGRG2

    Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00

    page-FLP100106 MFSD13A Fig.2 SDS PAGE 1

    Full Length Transmembrane Proteins

    Human MFSD13A full length protein-synthetic nanodisc

    SKU:  FLP100106     Target:  MFSD13A

    Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00

    elisa-FLP100105 MRGPRX2 Fig.1 Elisa 1

    Full Length Transmembrane Proteins

    Human MRGPRX2 full length protein-synthetic nanodisc

    SKU:  FLP100105     Target:  MRGPRX2

    Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00

    elisa-FLP100104 ADGRG1 Fig.1 Elisa 1

    Full Length Transmembrane Proteins

    Human ADGRG1 full length protein-synthetic nanodisc

    SKU:  FLP100104     Target:  ADGRG1

    Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00

    page-flp100103 gpr65 sp1

    Full Length Transmembrane Proteins

    Human GPR65 full length protein-synthetic nanodisc

    SKU:  FLP100103     Target:  GPR65

    Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00

    elisa-FLP100102 PROKR1 Fig.1 Elisa 1

    Full Length Transmembrane Proteins

    Human PROKR1 full length protein-synthetic nanodisc

    SKU:  FLP100102     Target:  PROKR1

    Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00

    elisa-FLP100101 TMCC3 Fig.1 Elisa 1

    Full Length Transmembrane Proteins

    Human TMCC3 full length protein-synthetic nanodisc

    SKU:  FLP100101     Target:  TMCC3

    Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00

    page-FLP100100 CCR5 Fig.2 SDS PAGE 1

    Full Length Transmembrane Proteins

    Human CCR5 full length protein-synthetic nanodisc

    SKU:  FLP100100     Target:  CCR5

    Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00

    elisa-FLP100099 HCRTR1 Fig.1 Elisa 1

    Full Length Transmembrane Proteins

    Human HCRTR1 full length protein-synthetic nanodisc

    SKU:  FLP100099     Target:  HCRTR1

    Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00

    elisa-FLP100097 PTGER4 Fig.1 Elisa 1

    Full Length Transmembrane Proteins

    Human PTGER4 full length protein-synthetic nanodisc

    SKU:  FLP100097     Target:  PTGER4

    Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00

    page-flp100096 ptger2 sp1

    Full Length Transmembrane Proteins

    Human PTGER2 full length protein-synthetic nanodisc

    SKU:  FLP100096     Target:  PTGER2

    Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00