Tag Free Recombinant Protein Service

Tag-free proteins are essential for various research applications. They closely mimic their native forms, allowing for more accurate studies of biological functions and interactions. This is particularly important in fields like drug development, vaccine research, and structural biology.

Purifying tag-free proteins presents unique challenges:

Lack of Specific Antibodies: Unlike tagged proteins, tag-free proteins do not have established affinity purification methods.
Stability and Solubility Issues: Without tags, some proteins can be less stable and soluble, which complicates the purification process.

At DIMA, we employ a cleavable tag system that combines the benefits of tagged proteins with the final advantages of tag-free proteins. Our approach includes fusion tags for better expression and purification efficiency, and protease system for precise cleavage, ensuring minimal impact on protein functionality.

Advantages of Untagged Proteins

Native Structure: Retaining their natural conformation, untagged proteins are better for studying protein interactions and enzyme activities.
Better Functionality: Untagged proteins preserve their natural biological activity, leading to more reliable results.
Versatile Applications: Ideal for drug development, vaccine research, structural biology, and high-throughput screening.

Workflow

Proteases for Tag Cleavage

Protease	Cleavage Site	Note
HRV 3C Protease	LEVLFQ/GP	Widely used for its specificity and efficiency.
Enterokinase	X-Asp-Asp-Asp-Asp-Lys-â-Not Proline-X	Ideal for removing tags like the FLAG tag.
SUMO Protease	Recognize the tertiary structure of SUMO	Highly specific for the SUMO protein fusion,
TEV Protease	ENLYFQG/S	Known for high specificity.
Thrombin	LVPR/GS	Commonly used in many expression systems.
Factor Xa	IEGR or IDGR	cleavage site can easily be inserted between a tag sequence and the protein of interest.

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Highlights

Mammalian Cell Expression: Ensures proper folding and post-translational modifications for functional proteins.
Efficient Purification: Uses anti-tag antibodies for high-yield purification.
Cleavable Tag Technology: Easily removes tags after purification, providing pure, untagged proteins.
Simplified Process: Cleavable tag and affinity purification streamline the process, saving time and resources—ideal for high-throughput and large-scale production.
High Success Rate: Delivers consistent, high-quality results.
Membrane Protein Expertise: Specializes in challenging membrane proteins and drug targets.

Case Studies

Testmonial

Dr. Chen, Senior Scientist

We have been using DIMA’s protein preparation service for several projects, and the results have been outstanding. The proteins were not only high quality but also maintained their biological activity, which is essential for our assays. The fast turnaround time and the expertise of their staff made collaboration easy and efficient. I highly recommend their services to any researcher looking for reliable and efficient protein preparation.