| Tag | C-Flag&Strep Tag |
|---|---|
| Target | |
| Synonyms | CRF-R; CRF-R-1; CRF-R1; CRF1; CRFR-1; CRFR1; CRH-R-1; CRH-R1; CRHR; CRHR1L |
| Description | Human CRHR1-Strep full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | P34998 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Long-term depression, Neuroactive ligand-receptor interaction |
| Molecular Weight | The human full length CRHR1-Strep protein has a MW of 47.7 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | A G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human CRHR1-Strep full length protein-synthetic nanodisc
Price: 10 μg $810.00 ; 50 μg $3960.00 ; 100 μg $7200.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag CRHR1-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with CRHR1-Strep-nanodisc is 6.581ng/ml.
Figure 2. Human CRHR1-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
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